Anders Benteson Nygaard's public defense of his thesis at the Ph.D programme in Health Sciences. Thesis title: Exploring the Built Environment Microbiomes of Norwegian Kindergartens and Nursing Homes
Wednesday April 3 2019, at 10:00
Title: Current knowledge on environmental microbial exposure in early life and the development of asthma and allergy
The candidate will defend his thesis on April 3 2019 at 12:15
We ask the audience to take their seats in good time before the public defense commences.
- First opponent: Senior researcher Martin Taübel, Nationel Institute for Health and Welfare, Finland
- Second opponent: Professor Maria Jenmalm, Linkoping University, Sweden
- Leader of the Committee: Associate Professor Yuri Kiselev, OsloMet, Norway
Leader of the public defence
To be announced
- Main supervisor: Professor Colin Charnock, OsloMet, Norway
- Co-supervisor: Professor Oddbjørn Sjøvold, NTNU, Norway
People spend much of the day indoors. Here they are exposed to the microorganisms that are present in buildings, often referred to as the indoor microbiome. This contact is now known to have relevance for human health and development. The aim of this thesis was to provide new insights into the structure and significance of indoor microbiomes in Norwegian kindergartens and nursing homes, based on modern high-throughput sequencing (HTS) and supplementary analyses. HTS-technologies are enabling unprecedented insights into microbial communities.
Materials and methods
Analyses was performed on ventilation filter dust and floor dust samples collected from Norwegian kindergartens and nursing homes. Dust samples were analyzed for microbial content by sequencing 16S rRNA gene amplicons on several HTS-platforms. In addition, dust samples were analyzed using supplementary culture-, endotoxin-, and antibiotic resistance genes (ARG)-analyses.
It was found that ventilation exhaust-filter dust and indoor floor dust samples had high proportions of human-associated bacterial taxa, some of clinical significance, such as Propionibacterium, Streptococcus, Staphylococcus, and Corynebacterium. In addition, the results of culture-, endotoxin-, and ARG-analyses provided valuable information to supplement the results generated by HTS. Further, it was found that for classification of indoor microbial communities, the Oxford Nanopore Technologies (ONT) MinION was able to provide greater taxonomic resolution than Illumina MiSeq, particularly at the species level.
The works presented in this thesis taken together, show that indoor microbial communities in kindergartens and nursing homes as revealed by the HTS-approach, are clearly structured by human occupancy. Furthermore, the newest addition to the HTS-market, the ONT MinION sequencing platform, provided good taxonomic assignments, by comparison with the Illumina MiSeq platform. This suggests a potential for future use of ONT MinION for studies of indoor microbial communities.