Epigenetic regulation by Atlantic salmon miRNAs in disease and osmotic stress

This project aims to provide new knowledge on Atlantic salmon miRNAs as regulators of immune responses to other pathogens as well as stress related to sea water transfer.

MicroRNAs are gene expression regulators pivotal in pathogen response and in adaptation to new environmental conditions in vertebrates. We have previously characterized all known miRNA genes in Atlantic salmon.

Here, we identify those that regulate immune responses as well as stress related to sea water transfer with modern functional genomics, transcriptomics and proteomics methods.

Disease constitutes a major challenge for the aquaculture industry and causes large economic losses. In order to improve health and reduce the negative effect of bacterial and parasite disease as well as stress related to smoltification and sea water transfer (SWT), more knowledge about disease mechanisms is necessary.

We apply modern functional genomics methods like small-RNA sequencing, RT-qPCR, and in vitro assays developed by our group to identify which miRNA genes regulate immune and stress responses. Furthermore we identify the genes regulated by these miRNAs (target genes) applying RNA-protein precipitation methods to understand what gene pathways are affected.

The pathogen model systems investigated includes Moritella viscosa (winter ulcer), Paramoeba perurans (gill disease) and ISAV (infectious salmon anaemia).

The results will disclose molecular details of disease and stress mechanisms and contribute novel knowledge that can help improve fish health, make changes in management and provide biomarkers to monitor fish health.